Hotspot

ndhE

SSC · 122645-122950 · 306 bp

Back to Hyoscyamus

Pi 0.475

MarkerSeek score 76.10

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 131
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CTATTTTGTAGCTGCTGCGC	CGTGGAGCAGAAGCAATTTG	50.0	50.0	57.9	58.0	1214 bp	0.5	59.0
ndhE_p2	CTATTTTGTAGCTGCTGCGC	TCGTGGAGCAGAAGCAATTT	50.0	45.0	57.9	58.1	1215 bp	0.5	58.4
ndhE_p3	CTATTTTGTAGCTGCTGCGC	TTCGTGGAGCAGAAGCAATT	50.0	45.0	57.9	58.1	1216 bp	0.5	58.4
ndhE_p4	CTATTTTGTAGCTGCTGCGC	GAGCACAGGTTTGTCGGTAA	50.0	50.0	57.9	58.1	1389 bp	0.5	58.2
ndhE_p5	CTATTTTGTAGCTGCTGCGC	AGAAATCAGTTGGACAGGCG	50.0	50.0	57.9	58.2	1270 bp	0.5	57.7