Hotspot

ndhD

SSC · 123566-125068 · 1503 bp

Back to Hyoscyamus

Pi 0.467

MarkerSeek score 80.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 578
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAAATTGCTTCTGCTCCACG	CATTGTATGAACCAGCGTGC	50.0	50.0	58.0	57.8	1956 bp	0.5	58.4
ndhD_p2	CAAATTGCTTCTGCTCCACG	GCATTGTATGAACCAGCGTG	50.0	50.0	58.0	57.8	1957 bp	0.5	58.4
ndhD_p3	CAAATTGCTTCTGCTCCACG	ATTCTGCAATTGTAGCGGCT	50.0	45.0	58.0	58.2	2103 bp	0.5	57.8
ndhD_p4	AATTGCTTCTGCTCCACGAA	CATTGTATGAACCAGCGTGC	45.0	50.0	58.1	57.8	1954 bp	0.5	57.7
ndhD_p5	AATTGCTTCTGCTCCACGAA	GCATTGTATGAACCAGCGTG	45.0	50.0	58.1	57.8	1955 bp	0.5	57.7