Hotspot

ndhA

SSC · 118739-120953 · 2215 bp

Back to Hyoscyamus

Pi 0.48

MarkerSeek score 80.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2215
variable_sites: 954
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTGAGAGTTGAAGCCCCAAA	TCAAAGTGTATTCGGCCTCG	50.0	50.0	58.0	58.0	2680 bp	0.5	59.8
ndhA_p2	GCAACGAGAAGGGGATTCAT	TCAAAGTGTATTCGGCCTCG	50.0	50.0	58.0	58.0	2909 bp	0.5	59.6
ndhA_p3	CAGGCTCTGGAAGGAATTCC	TCAAAGTGTATTCGGCCTCG	55.0	50.0	58.0	58.0	2833 bp	0.5	59.6
ndhA_p4	GTGAGAGTTGAAGCCCCAAA	CGTGGGACAGGGTAATCATG	50.0	55.0	58.0	58.0	2598 bp	0.5	59.5
ndhA_p5	GCAACGAGAAGGGGATTCAT	CGTGGGACAGGGTAATCATG	50.0	55.0	58.0	58.0	2827 bp	0.5	59.3