Hotspot

ccsA

SSC · 125300-126241 · 942 bp

Back to Hyoscyamus

Pi 0.435

MarkerSeek score 77.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 942
variable_sites: 298
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TATCTTGACGAAATGGGCGG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1773 bp	0.5	59.5
ccsA_p2	TGACGAAATGGGCGGAATAG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1768 bp	0.5	59.5
ccsA_p3	TATCTTGACGAAATGGGCGG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.1	58.0	1772 bp	0.5	59.5
ccsA_p4	TGACGAAATGGGCGGAATAG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.1	58.0	1767 bp	0.5	59.5
ccsA_p5	TTTTTGATTCTGGACCGCGA	GTGAAATCGGTAGACACGCT	45.0	50.0	58.1	58.0	1490 bp	0.5	59.0