Hotspot

trnL(UAG)-rpl32

SSC · 126425-127304 · 880 bp

Back to Hyoscyamus

Pi 0.449

MarkerSeek score 80.90

Primer available yes

Label trnL(UAG)-rpl32

Feature Metadata

label_name: trnL(UAG)-rpl32
feature_type: igs
region: SSC
length_bp: 880
variable_sites: 338
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(UAG)-rpl32_p1	AGCGTGTCTACCGATTTCAC	CTCCTTTGAAATAGGCCCGT	50.0	50.0	58.0	57.6	1613 bp	0.5	56.6
trnL(UAG)-rpl32_p2	CAGCGTGTCTACCGATTTCA	CTCCTTTGAAATAGGCCCGT	50.0	50.0	58.0	57.6	1614 bp	0.5	56.6
trnL(UAG)-rpl32_p3	AGCGTGTCTACCGATTTCAC	TGAGCAGTGAATCAAACGGG	50.0	50.0	58.0	58.5	1579 bp	0.5	56.1
trnL(UAG)-rpl32_p4	CAGCGTGTCTACCGATTTCA	TGAGCAGTGAATCAAACGGG	50.0	50.0	58.0	58.5	1580 bp	0.5	56.1
trnL(UAG)-rpl32_p5	GAAGTAGCAAAGCCTTGGGT	CTCCTTTGAAATAGGCCCGT	50.0	50.0	58.1	57.6	2111 bp	0.5	55.9