Hotspot

ndhI-ndhG

SSC · 121542-121954 · 413 bp

Back to Hyoscyamus

Pi 0.44

MarkerSeek score 75.90

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 148
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CGAGGCCGAATACACTTTGA	TCCATCTCCTACGGTCCAAA	50.0	50.0	58.0	57.8	1052 bp	0.5	58.0
ndhI-ndhG_p2	CGAGGCCGAATACACTTTGA	TTCCATCTCCTACGGTCCAA	50.0	50.0	58.0	57.8	1053 bp	0.5	58.0
ndhI-ndhG_p3	CATGATTACCCTGTCCCACG	TCCATCTCCTACGGTCCAAA	55.0	50.0	58.0	57.8	1134 bp	0.5	57.7
ndhI-ndhG_p4	CATGATTACCCTGTCCCACG	TTCCATCTCCTACGGTCCAA	55.0	50.0	58.0	57.8	1135 bp	0.5	57.7
ndhI-ndhG_p5	GATGGACTTTACCGAGGCTG	TCCATCTCCTACGGTCCAAA	55.0	50.0	58.1	57.8	1414 bp	0.5	57.6