primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	ACACTCGGAATATTTCATTCGGA	TGGGCGCGTTGGAATTATAA	23	20	39.1	45.0	58.0	58.0	0.0	8.4	3.058357	114910	115029	421-421	0.500000	35.5
ycf1_p2	ycf1	2	ACACTCGGAATATTTCATTCGGA	TTGGGCGCGTTGGAATTATA	23	20	39.1	45.0	58.0	58.0	0.0	8.4	3.058357	114910	115029	422-422	0.500000	35.5
ycf1_p3	ycf1	3	ACACTCGGAATATTTCATTCGGA	ATCGATCTTAACCCAGGCCT	23	20	39.1	50.0	58.0	58.2	0.0	0.0	3.210725	114910	115029	588-588	0.500000	34.3
ycf1_p4	ycf1	4	ACACTCGGAATATTTCATTCGGA	GGATCACCGGGCATATCAAA	23	20	39.1	50.0	58.0	57.7	0.0	6.3	3.288891	114910	115029	452-452	0.500000	33.7
ycf1_p5	ycf1	5	ACACTCGGAATATTTCATTCGG	TGGGCGCGTTGGAATTATAA	22	20	40.9	45.0	56.6	58.0	0.0	8.4	3.445129	114910	115029	421-421	0.500000	32.4
ycf1-rps15_p1	ycf1-rps15	1	TGAATTGGAATGGCGTCGAT	AGTCGTTGACGTTTTCCCAA	20	20	45.0	45.0	58.0	58.0	0.0	3.2	0.063961	116777	117172	829-829	0.500000	59.5
ycf1-rps15_p2	ycf1-rps15	2	TGAATTGGAATGGCGTCGAT	GCCTAATTGTTCGGGTCCAT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.089740	116777	117172	1303-1303	0.500000	59.3
ycf1-rps15_p3	ycf1-rps15	3	TGAATTGGAATGGCGTCGAT	ATGAGTCGAAGAACACCGTG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.102950	116777	117172	1119-1119	0.500000	59.2
ycf1-rps15_p4	ycf1-rps15	4	TGAATTGGAATGGCGTCGAT	CCAACGTGTTACATAGGGCA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.139027	116777	117172	1243-1243	0.500000	58.9
ycf1-rps15_p5	ycf1-rps15	5	TGAATTGGAATGGCGTCGAT	AGCTATCCGACTCAACTCCA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.182354	116777	117172	1369-1369	0.500000	58.5
ndhH_p1	ndhH	1	TTGGGAAAACGTCAACGACT	GAAGTCCAACAGGAGCAACA	20	20	45.0	50.0	58.0	58.0	9.9	0.0	0.059685	117556	118737	1843-1843	0.500000	59.5
ndhH_p2	ndhH	2	TTGGGAAAACGTCAACGACT	CACCAATGCTAAGATCGGCT	20	20	45.0	50.0	58.0	58.0	9.9	9.1	0.067962	117556	118737	1798-1798	0.500000	59.5
ndhH_p3	ndhH	3	TTGGGAAAACGTCAACGACT	ATTCAGGTCCGATACGTTGC	20	20	45.0	50.0	58.0	58.1	9.9	0.0	0.091071	117556	118737	1603-1603	0.500000	59.3
ndhH_p4	ndhH	4	TTGGGAAAACGTCAACGACT	GGAGCAACACTTGAAATGGC	20	20	45.0	50.0	58.0	57.9	9.9	0.0	0.097265	117556	118737	1832-1832	0.500000	59.2
ndhH_p5	ndhH	5	TTGGGAAAACGTCAACGACT	AAAGGGCCAGCATATTCAGG	20	20	45.0	50.0	58.0	57.9	9.9	6.2	0.104691	117556	118737	1616-1616	0.500000	59.2
ndhA_p1	ndhA	1	GTGAGAGTTGAAGCCCCAAA	TCAAAGTGTATTCGGCCTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023326	118739	120953	2680-2680	0.500000	59.8
ndhA_p2	ndhA	2	GCAACGAGAAGGGGATTCAT	TCAAAGTGTATTCGGCCTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048705	118739	120953	2909-2909	0.500000	59.6
ndhA_p3	ndhA	3	CAGGCTCTGGAAGGAATTCC	TCAAAGTGTATTCGGCCTCG	20	20	55.0	50.0	58.0	58.0	17.3	0.0	0.054471	118739	120953	2833-2833	0.500000	59.6
ndhA_p4	ndhA	4	GTGAGAGTTGAAGCCCCAAA	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.065185	118739	120953	2598-2598	0.500000	59.5
ndhA_p5	ndhA	5	GCAACGAGAAGGGGATTCAT	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.090564	118739	120953	2827-2827	0.500000	59.3
ndhI_p1	ndhI	1	CGATGGGCACAGTGATGTTA	TCCATCTCCTACGGTCCAAA	20	20	50.0	50.0	58.0	57.8	4.1	0.0	0.264045	121038	121541	1868-1868	0.500000	57.9
ndhI_p2	ndhI	2	CGATGGGCACAGTGATGTTA	TTCCATCTCCTACGGTCCAA	20	20	50.0	50.0	58.0	57.8	4.1	0.0	0.264045	121038	121541	1869-1869	0.500000	57.9
ndhI_p3	ndhI	3	GATGGACTTTACCGAGGCTG	TCCATCTCCTACGGTCCAAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.299956	121038	121541	1414-1414	0.500000	57.6
ndhI_p4	ndhI	4	GATGGACTTTACCGAGGCTG	TTCCATCTCCTACGGTCCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.299956	121038	121541	1415-1415	0.500000	57.6
ndhI_p5	ndhI	5	AGATGGACTTTACCGAGGCT	TCCATCTCCTACGGTCCAAA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.373918	121038	121541	1415-1415	0.500000	57.0
ndhI-ndhG_p1	ndhI-ndhG	1	CGAGGCCGAATACACTTTGA	TCCATCTCCTACGGTCCAAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245863	121542	121954	1052-1052	0.500000	58.0
ndhI-ndhG_p2	ndhI-ndhG	2	CGAGGCCGAATACACTTTGA	TTCCATCTCCTACGGTCCAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245863	121542	121954	1053-1053	0.500000	58.0
ndhI-ndhG_p3	ndhI-ndhG	3	CATGATTACCCTGTCCCACG	TCCATCTCCTACGGTCCAAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.287722	121542	121954	1134-1134	0.500000	57.7
ndhI-ndhG_p4	ndhI-ndhG	4	CATGATTACCCTGTCCCACG	TTCCATCTCCTACGGTCCAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.287722	121542	121954	1135-1135	0.500000	57.7
ndhI-ndhG_p5	ndhI-ndhG	5	GATGGACTTTACCGAGGCTG	TCCATCTCCTACGGTCCAAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.299956	121542	121954	1414-1414	0.500000	57.6
ndhG-ndhE_p1	ndhG-ndhE	1	CTATTTTGTAGCTGCTGCGC	CGTGGAGCAGAAGCAATTTG	20	20	50.0	50.0	57.9	58.0	13.4	0.0	0.123661	122486	122644	1214-1214	0.500000	59.0
ndhG-ndhE_p2	ndhG-ndhE	2	CTATTTTGTAGCTGCTGCGC	TCGTGGAGCAGAAGCAATTT	20	20	50.0	45.0	57.9	58.1	13.4	0.0	0.200579	122486	122644	1215-1215	0.500000	58.4
ndhG-ndhE_p3	ndhG-ndhE	3	CTATTTTGTAGCTGCTGCGC	TTCGTGGAGCAGAAGCAATT	20	20	50.0	45.0	57.9	58.1	13.4	0.0	0.200579	122486	122644	1216-1216	0.500000	58.4
ndhG-ndhE_p4	ndhG-ndhE	4	CTATTTTGTAGCTGCTGCGC	AGAAATCAGTTGGACAGGCG	20	20	50.0	50.0	57.9	58.2	13.4	0.0	0.283286	122486	122644	1270-1270	0.500000	57.7
ndhG-ndhE_p5	ndhG-ndhE	5	GCCTTTTCCTTGGGATTGGT	CGTGGAGCAGAAGCAATTTG	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.382353	122486	122644	1273-1273	0.500000	56.9
ndhE_p1	ndhE	1	CTATTTTGTAGCTGCTGCGC	CGTGGAGCAGAAGCAATTTG	20	20	50.0	50.0	57.9	58.0	13.4	0.0	0.123661	122645	122950	1214-1214	0.500000	59.0
ndhE_p2	ndhE	2	CTATTTTGTAGCTGCTGCGC	TCGTGGAGCAGAAGCAATTT	20	20	50.0	45.0	57.9	58.1	13.4	0.0	0.200579	122645	122950	1215-1215	0.500000	58.4
ndhE_p3	ndhE	3	CTATTTTGTAGCTGCTGCGC	TTCGTGGAGCAGAAGCAATT	20	20	50.0	45.0	57.9	58.1	13.4	0.0	0.200579	122645	122950	1216-1216	0.500000	58.4
ndhE_p4	ndhE	4	CTATTTTGTAGCTGCTGCGC	GAGCACAGGTTTGTCGGTAA	20	20	50.0	50.0	57.9	58.1	13.4	0.0	0.223101	122645	122950	1389-1389	0.500000	58.2
ndhE_p5	ndhE	5	CTATTTTGTAGCTGCTGCGC	AGAAATCAGTTGGACAGGCG	20	20	50.0	50.0	57.9	58.2	13.4	0.0	0.283286	122645	122950	1270-1270	0.500000	57.7
ndhE-psaC_p1	ndhE-psaC	1	AGGTGCAATTGCTGTAGCTC	CGTGGAGCAGAAGCAATTTG	20	20	50.0	50.0	58.5	58.0	29.4	0.0	0.569515	122951	123201	875-875	0.500000	55.4
ndhE-psaC_p2	ndhE-psaC	2	AGGTGCAATTGCTGTAGCTC	AAGCCGCTAAAGTCGCTAAA	20	20	50.0	45.0	58.5	57.9	29.4	0.0	0.631603	122951	123201	1421-1421	0.500000	54.9
ndhE-psaC_p3	ndhE-psaC	3	AGGTGCAATTGCTGTAGCTC	TCGTGGAGCAGAAGCAATTT	20	20	50.0	45.0	58.5	58.1	29.4	0.0	0.646434	122951	123201	876-876	0.500000	54.8
ndhE-psaC_p4	ndhE-psaC	4	AGGTGCAATTGCTGTAGCTC	TTCGTGGAGCAGAAGCAATT	20	20	50.0	45.0	58.5	58.1	29.4	0.0	0.646434	122951	123201	877-877	0.500000	54.8
ndhE-psaC_p5	ndhE-psaC	5	AGGTGCAATTGCTGTAGCTC	GAGCACAGGTTTGTCGGTAA	20	20	50.0	50.0	58.5	58.1	29.4	0.0	0.668956	122951	123201	1050-1050	0.500000	54.6
psaC_p1	psaC	1	AGGTGCAATTGCTGTAGCTC	AAGAGAAAAACGGAACCCCC	20	20	50.0	50.0	58.5	58.0	29.4	0.0	0.551533	123202	123447	1644-1644	0.500000	55.6
psaC_p2	psaC	2	AGGTGCAATTGCTGTAGCTC	AGTAAAAGGTCCCGGGATGA	20	20	50.0	50.0	58.5	58.0	29.4	29.7	0.581521	123202	123447	1515-1515	0.500000	55.3
psaC_p3	psaC	3	AGGTGCAATTGCTGTAGCTC	AAGCCGCTAAAGTCGCTAAA	20	20	50.0	45.0	58.5	57.9	29.4	0.0	0.631603	123202	123447	1421-1421	0.500000	54.9
psaC_p4	psaC	4	AGGTGCAATTGCTGTAGCTC	CCCCTGCCGTGTACAAAATA	20	20	50.0	50.0	58.5	57.9	29.4	0.0	0.659481	123202	123447	1628-1628	0.500000	54.7
psaC_p5	psaC	5	AGGTGCAATTGCTGTAGCTC	GAGCACAGGTTTGTCGGTAA	20	20	50.0	50.0	58.5	58.1	29.4	0.0	0.668956	123202	123447	1050-1050	0.500000	54.6
ndhD_p1	ndhD	1	CAAATTGCTTCTGCTCCACG	CATTGTATGAACCAGCGTGC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.205336	123566	125068	1956-1956	0.500000	58.4
ndhD_p2	ndhD	2	CAAATTGCTTCTGCTCCACG	GCATTGTATGAACCAGCGTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.205336	123566	125068	1957-1957	0.500000	58.4
ndhD_p3	ndhD	3	CAAATTGCTTCTGCTCCACG	ATTCTGCAATTGTAGCGGCT	20	20	50.0	45.0	58.0	58.2	0.0	17.8	0.271682	123566	125068	2103-2103	0.500000	57.8
ndhD_p4	ndhD	4	AATTGCTTCTGCTCCACGAA	CATTGTATGAACCAGCGTGC	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.282254	123566	125068	1954-1954	0.500000	57.7
ndhD_p5	ndhD	5	AATTGCTTCTGCTCCACGAA	GCATTGTATGAACCAGCGTG	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.282254	123566	125068	1955-1955	0.500000	57.7
ndhD-ccsA_p1	ndhD-ccsA	1	TATCTTGACGAAATGGGCGG	ACCCAAGGCTTTGCTACTTC	20	20	50.0	50.0	58.1	58.1	0.0	2.4	0.140838	125069	125299	1275-1275	0.500000	58.9
ndhD-ccsA_p2	ndhD-ccsA	2	TGACGAAATGGGCGGAATAG	ACCCAAGGCTTTGCTACTTC	20	20	50.0	50.0	58.1	58.1	0.0	2.4	0.140838	125069	125299	1270-1270	0.500000	58.9
ndhD-ccsA_p3	ndhD-ccsA	3	TATCTTGACGAAATGGGCGG	CCCAAGGCTTTGCTACTTCA	20	20	50.0	50.0	58.1	58.1	0.0	2.4	0.142196	125069	125299	1274-1274	0.500000	58.9
ndhD-ccsA_p4	ndhD-ccsA	4	TGACGAAATGGGCGGAATAG	CCCAAGGCTTTGCTACTTCA	20	20	50.0	50.0	58.1	58.1	0.0	2.4	0.142196	125069	125299	1269-1269	0.500000	58.9
ndhD-ccsA_p5	ndhD-ccsA	5	TGACGAAATGGGCGGAATAG	GCTCTTCAATCCGAGTGGTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.152500	125069	125299	1207-1207	0.500000	58.8
ccsA_p1	ccsA	1	TATCTTGACGAAATGGGCGG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.059726	125300	126241	1773-1773	0.500000	59.5
ccsA_p2	ccsA	2	TGACGAAATGGGCGGAATAG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.059726	125300	126241	1768-1768	0.500000	59.5
ccsA_p3	ccsA	3	TATCTTGACGAAATGGGCGG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.061097	125300	126241	1772-1772	0.500000	59.5
ccsA_p4	ccsA	4	TGACGAAATGGGCGGAATAG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.061097	125300	126241	1767-1767	0.500000	59.5
ccsA_p5	ccsA	5	TTTTTGATTCTGGACCGCGA	GTGAAATCGGTAGACACGCT	20	20	45.0	50.0	58.1	58.0	8.2	0.0	0.122270	125300	126241	1490-1490	0.500000	59.0
trnL(UAG)-rpl32_p1	trnL(UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	CTCCTTTGAAATAGGCCCGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.427889	126425	127304	1613-1613	0.500000	56.6
trnL(UAG)-rpl32_p2	trnL(UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	CTCCTTTGAAATAGGCCCGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.429259	126425	127304	1614-1614	0.500000	56.6
trnL(UAG)-rpl32_p3	trnL(UAG)-rpl32	3	AGCGTGTCTACCGATTTCAC	TGAGCAGTGAATCAAACGGG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.485162	126425	127304	1579-1579	0.500000	56.1
trnL(UAG)-rpl32_p4	trnL(UAG)-rpl32	4	CAGCGTGTCTACCGATTTCA	TGAGCAGTGAATCAAACGGG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.486532	126425	127304	1580-1580	0.500000	56.1
trnL(UAG)-rpl32_p5	trnL(UAG)-rpl32	5	GAAGTAGCAAAGCCTTGGGT	CTCCTTTGAAATAGGCCCGT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.509001	126425	127304	2111-2111	0.500000	55.9
rpl32_p1	rpl32	1	ACTCCCCACCTTGGAATAGA	CTCCTTTGAAATAGGCCCGT	20	20	50.0	50.0	57.3	57.6	0.0	0.0	1.081235	127305	127472	953-953	0.500000	51.4
rpl32_p2	rpl32	2	ACTCCCCACCTTGGAATAGA	TGAGCAGTGAATCAAACGGG	20	20	50.0	50.0	57.3	58.5	0.0	0.0	1.138508	127305	127472	919-919	0.500000	50.9
rpl32_p3	rpl32	3	ACTCCCCACCTTGGAATAGA	GCAGTGAATCAAACGGGGAT	20	20	50.0	50.0	57.3	58.5	0.0	0.0	1.197502	127305	127472	916-916	0.500000	50.4
rpl32_p4	rpl32	4	ACTCCCCACCTTGGAATAGA	TCTCCTTTGAAATAGGCCCG	20	20	50.0	50.0	57.3	57.3	0.0	0.0	1.371272	127305	127472	954-954	0.500000	49.0
rpl32_p5	rpl32	5	ACTCCCCACCTTGGAATAGAA	CTCCTTTGAAATAGGCCCGT	21	20	47.6	50.0	58.1	57.6	0.0	0.0	1.490758	127305	127472	953-953	0.500000	48.1
rpl32-ndhF_p1	rpl32-ndhF	1	ACTCCCCACCTTGGAATAGA	AACGGGTCGTGTAAACCAAA	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.696706	127473	128238	1693-1693	0.500000	54.4
rpl32-ndhF_p2	rpl32-ndhF	2	ACTCCCCACCTTGGAATAGA	ACGGGTCGTGTAAACCAAAA	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.696706	127473	128238	1692-1692	0.500000	54.4
rpl32-ndhF_p3	rpl32-ndhF	3	ACTCCCCACCTTGGAATAGA	TGGGTCGATCAAGTATCCGA	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.722686	127473	128238	1456-1456	0.500000	54.2
rpl32-ndhF_p4	rpl32-ndhF	4	ACTCCCCACCTTGGAATAGA	TCTGAAAAGCCCACATACGG	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.769126	127473	128238	1313-1313	0.500000	53.8
rpl32-ndhF_p5	rpl32-ndhF	5	ACTCCCCACCTTGGAATAGA	GTGGGTCGATCAAGTATCCG	20	20	50.0	55.0	57.3	57.9	0.0	0.0	0.796840	127473	128238	1457-1457	0.500000	53.6
ndhF_p1	ndhF	1	ACGGGCCTATTTCAAAGGAG	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	57.6	57.7	0.8	1.8	0.752122	128239	130461	2778-2778	0.500000	54.0
ndhF_p2	ndhF	2	CCCGTTTGATTCACTGCTCA	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	58.5	57.7	0.0	1.8	0.809395	128239	130461	2812-2812	0.500000	53.5
ndhF_p3	ndhF	3	ATCCCCGTTTGATTCACTGC	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	58.5	57.7	0.0	1.8	0.868389	128239	130461	2815-2815	0.500000	53.1
ndhF_p4	ndhF	4	ACGGGCCTATTTCAAAGGAG	TGAACTGGACAATCAGTGGG	20	20	50.0	50.0	57.6	57.4	0.8	0.0	0.986892	128239	130461	2577-2577	0.500000	52.1
ndhF_p5	ndhF	5	ACGGGCCTATTTCAAAGGAG	GAACTGGACAATCAGTGGGT	20	20	50.0	50.0	57.6	57.4	0.8	0.0	0.988681	128239	130461	2576-2576	0.500000	52.1