Hotspot

psbA

LSC · 111-1172 · 1062 bp

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Pi 0

MarkerSeek score 28.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGCTCCATTCCTTCAACGA	GTGCTTGGGAGTCCTTGAAA	45.0	50.0	57.4	58.0	1149 bp	1.0	75.4
psbA_p2	TTGCTCCATTCCTTCAACGA	AATACGTGTGCTTGGGAGTC	45.0	50.0	57.4	57.9	1156 bp	1.0	74.8
psbA_p3	TGCTCCATTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	45.0	50.0	57.2	58.0	1148 bp	1.0	73.6
psbA_p4	TGCTCCATTCCTTCAACGAT	AATACGTGTGCTTGGGAGTC	45.0	50.0	57.2	57.9	1155 bp	1.0	72.9
psbA_p5	TGCTCCATTCCTTCAACGATT	GTGCTTGGGAGTCCTTGAAA	42.9	50.0	57.9	58.0	1148 bp	1.0	71.3