Hotspot

ndhD-psaC

SSC · 119203-119413 · 211 bp

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Pi 0.0332

MarkerSeek score 71.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 211
variable_sites: 7
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGGATCGTCCAATTGGAAATGA	AACCGAGGACTGTGTAGGTT	40.9	50.0	58.0	58.3	416 bp	1.0	62.7
ndhD-psaC_p2	TGGATCGTCCAATTGGAAATGA	AAGAGATGTGAATCCGCCTG	40.9	50.0	58.0	57.7	394 bp	1.0	62.5
ndhD-psaC_p3	TGGATCGTCCAATTGGAAATGA	AGAACCGAGGACTGTGTAGG	40.9	55.0	58.0	58.5	418 bp	1.0	61.4
ndhD-psaC_p4	TGGATCGTCCAATTGGAAATGA	ATGAAACAACTCGCAGCATG	40.9	45.0	58.0	57.4	336 bp	1.0	60.4
ndhD-psaC_p5	TGGATCGTCCAATTGGAAATGA	CATGAAACAACTCGCAGCAT	40.9	45.0	58.0	57.4	337 bp	1.0	60.4