Hotspot

trnF-GAA-ndhJ

LSC · 49470-50221 · 752 bp

Back to Hyophorbe

Pi 0.00266

MarkerSeek score 60.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 2
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	862 bp	1.0	78.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	852 bp	1.0	78.2
trnF-GAA-ndhJ_p3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.8	57.9	908 bp	1.0	77.9
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	905 bp	1.0	77.6
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	904 bp	1.0	77.6