Hotspot

ycf3-trnS-UGA

LSC · 45465-46084 · 620 bp

Back to Hyophorbe

Pi 0.00323

MarkerSeek score 60.60

Primer available yes

Label ycf3-trnS-UGA

Feature Metadata

label_name: ycf3-trnS-UGA
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 2
pi: 0.00323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	783 bp	1.0	66.8
ycf3-trnS-UGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	784 bp	1.0	66.8
ycf3-trnS-UGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	804 bp	1.0	66.3
ycf3-trnS-UGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	805 bp	1.0	66.3
ycf3-trnS-UGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	754 bp	1.0	66.0