Hotspot

trnT-GGU-psbD

LSC · 32919-33957 · 1039 bp

Back to Hyophorbe

Pi 0.00289

MarkerSeek score 60.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 3
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1208-1222 bp	1.0	77.5
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1203-1217 bp	1.0	77.5
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1192-1206 bp	1.0	77.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1187-1201 bp	1.0	77.4
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1221-1235 bp	1.0	76.4