Hotspot

rps16-trnQ-UUG

LSC · 5914-7015 · 1102 bp

Back to Hyophorbe

Pi 0.00272
MarkerSeek score 60.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1102
variable_sites
3
pi
0.00272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1192-1193 bp 1.0 79.4
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1190-1191 bp 1.0 79.4
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1198-1199 bp 1.0 79.3
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1199-1200 bp 1.0 79.3
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA GAAATTCGAAATGGGGCGTG 50.0 50.0 58.0 58.1 1251-1252 bp 1.0 79.3