primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TTGCTCCATTCCTTCAACGA	GTGCTTGGGAGTCCTTGAAA	20	20	45.0	50.0	57.4	58.0	0.0	9.6	0.578441	111	1172	1149-1149	1.000000	75.4
psbA_p2	psbA	2	TTGCTCCATTCCTTCAACGA	AATACGTGTGCTTGGGAGTC	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.654460	111	1172	1156-1156	1.000000	74.8
psbA_p3	psbA	3	TGCTCCATTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	20	20	45.0	50.0	57.2	58.0	0.0	9.6	0.806216	111	1172	1148-1148	1.000000	73.6
psbA_p4	psbA	4	TGCTCCATTCCTTCAACGAT	AATACGTGTGCTTGGGAGTC	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.882235	111	1172	1155-1155	1.000000	72.9
psbA_p5	psbA	5	TGCTCCATTCCTTCAACGATT	GTGCTTGGGAGTCCTTGAAA	21	20	42.9	50.0	57.9	58.0	0.0	9.6	1.090617	111	1172	1148-1148	1.000000	71.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	5914	7015	1192-1193	1.000000	79.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	5914	7015	1190-1191	1.000000	79.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5914	7015	1198-1199	1.000000	79.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5914	7015	1199-1200	1.000000	79.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAGCCGATCATGTCCTTCAA	GAAATTCGAAATGGGGCGTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120355	5914	7015	1251-1252	1.000000	79.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	AACGGAAAGACCCCTTAACT	20	20	45.0	45.0	58.0	56.4	0.0	0.0	1.674622	8342	9565	1329-1340	1.000000	67.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGAAGAAGCGGAGAGAGAGG	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.2	56.4	0.0	0.0	1.873708	8342	9565	1385-1396	1.000000	65.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AAACGGAAAGACCCCTTAACT	20	21	45.0	42.9	58.0	57.1	0.0	0.0	1.908745	8342	9565	1330-1341	1.000000	65.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGGAGAGAGAGGGATTCGAA	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.3	56.4	0.0	0.0	1.954878	8342	9565	1377-1388	1.000000	65.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	AACGGAAAGACCCCTTAACT	20	20	50.0	45.0	57.6	56.4	0.9	0.0	2.046364	8342	9565	1315-1326	1.000000	64.5
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	20	24	45.0	37.5	57.4	57.5	9.6	7.1	5.129437	12278	13635	1512-1517	1.000000	40.5
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	AGTCCTATCTATAAGAGGAGAGCA	20	24	45.0	41.7	57.4	57.3	9.6	0.0	5.354252	12278	13635	1494-1499	1.000000	40.5
atpF_p3	atpF	3	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	20	24	50.0	37.5	57.0	57.5	0.0	7.1	5.481484	12278	13635	1500-1505	1.000000	40.5
atpF_p4	atpF	4	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	20	23	45.0	39.1	57.4	56.2	9.6	7.1	5.482017	12278	13635	1512-1517	1.000000	40.5
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	20	23	45.0	39.1	57.4	56.2	9.6	3.5	5.482017	12278	13635	1511-1516	1.000000	40.5
rpoC1_p1	rpoC1	1	GAGAGACCCGAATCCCTACA	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	57.9	58.0	0.0	23.5	0.109087	21532	24339	2979-2987	1.000000	79.2
rpoC1_p2	rpoC1	2	AGAGACCCGAATCCCTACAG	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	57.9	58.0	0.0	23.5	0.114982	21532	24339	2978-2986	1.000000	79.2
rpoC1_p3	rpoC1	3	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	58.3	58.0	0.0	23.5	0.328197	21532	24339	2987-2995	1.000000	77.5
rpoC1_p4	rpoC1	4	GAGAGACCCGAATCCCTACA	GCTCGTTCGAGAACTACGAT	20	20	55.0	50.0	57.9	57.5	0.0	23.5	0.524129	21532	24339	2977-2985	1.000000	75.9
rpoC1_p5	rpoC1	5	AGAGACCCGAATCCCTACAG	GCTCGTTCGAGAACTACGAT	20	20	55.0	50.0	57.9	57.5	0.0	23.5	0.530023	21532	24339	2976-2984	1.000000	75.9
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	30018	30790	929-944	1.000000	68.5
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	30018	30790	924-939	1.000000	67.5
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30018	30790	874-889	1.000000	67.2
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	30018	30790	933-948	1.000000	65.5
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	30018	30790	932-947	1.000000	64.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	22.4	0.0	0.340432	32919	33957	1208-1222	1.000000	77.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	32919	33957	1203-1217	1.000000	77.5
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	20	20	50.0	55.0	58.1	57.7	22.4	0.0	0.350480	32919	33957	1192-1206	1.000000	77.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	20	20	50.0	55.0	57.9	57.7	0.0	0.0	0.359654	32919	33957	1187-1201	1.000000	77.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	20	20	50.0	50.0	58.1	58.4	22.4	0.0	0.478204	32919	33957	1221-1235	1.000000	76.4
ycf3-trnS-UGA_p1	ycf3-trnS-UGA	1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	45465	46084	783-783	1.000000	66.8
ycf3-trnS-UGA_p2	ycf3-trnS-UGA	2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	45465	46084	784-784	1.000000	66.8
ycf3-trnS-UGA_p3	ycf3-trnS-UGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45465	46084	804-804	1.000000	66.3
ycf3-trnS-UGA_p4	ycf3-trnS-UGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45465	46084	805-805	1.000000	66.3
ycf3-trnS-UGA_p5	ycf3-trnS-UGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	45465	46084	754-754	1.000000	66.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	47463	48465	1098-1115	1.000000	79.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	47463	48465	1106-1123	1.000000	78.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47463	48465	1085-1102	1.000000	78.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TCTAACCCCTGAGCTAAGCA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.252323	47463	48465	1046-1063	1.000000	78.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47463	48465	1093-1110	1.000000	77.8
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49470	50221	862-862	1.000000	78.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49470	50221	852-852	1.000000	78.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	49470	50221	908-908	1.000000	77.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49470	50221	905-905	1.000000	77.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49470	50221	904-904	1.000000	77.6
accD-psaI_p1	accD-psaI	1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60371	61220	958-960	1.000000	80.3
accD-psaI_p2	accD-psaI	2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60371	61220	959-961	1.000000	80.3
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60371	61220	964-966	1.000000	79.7
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60371	61220	965-967	1.000000	79.7
accD-psaI_p5	accD-psaI	5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	60371	61220	964-966	1.000000	79.6
rpl22-rps19_p1	rpl22-rps19	1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.5	58.0	8.2	9.6	0.525718	85830	85882	218-218	1.000000	78.0
rpl22-rps19_p2	rpl22-rps19	2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	20	20	50.0	55.0	58.5	57.8	8.2	9.6	0.689756	85830	85882	223-223	1.000000	76.6
rpl22-rps19_p3	rpl22-rps19	3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	20	20	50.0	50.0	58.5	57.7	8.2	9.6	0.778605	85830	85882	219-219	1.000000	76.0
rpl22-rps19_p4	rpl22-rps19	4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	20	20	50.0	50.0	58.5	58.5	8.2	17.5	0.959344	85830	85882	242-242	1.000000	74.3
rpl22-rps19_p5	rpl22-rps19	5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	20	20	50.0	45.0	58.5	58.5	8.2	9.6	1.000768	85830	85882	211-211	1.000000	74.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.630573	115650	116445	889-889	1.000000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.631944	115650	116445	890-890	1.000000	59.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	55.4	57.9	9.5	0.0	2.747020	115650	116445	899-899	1.000000	58.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	115650	116445	898-898	1.000000	57.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	115650	116445	897-897	1.000000	57.9
ndhD_p1	ndhD	1	TGCATTGTACAGCGAACGAT	ATTCGCGGGTTCCTCAATTT	20	20	45.0	45.0	58.3	58.2	0.0	6.1	0.445007	117790	119202	1540-1540	1.000000	76.4
ndhD_p2	ndhD	2	TTTGCATTGTACAGCGAACG	ATTCGCGGGTTCCTCAATTT	20	20	45.0	45.0	57.7	58.2	5.8	6.1	0.474159	117790	119202	1542-1542	1.000000	76.2
ndhD_p3	ndhD	3	TGCATTGTACAGCGAACGAT	GATATTCGCGGGTTCCTCAA	20	20	45.0	50.0	58.3	57.8	0.0	6.1	0.513495	117790	119202	1543-1543	1.000000	75.9
ndhD_p4	ndhD	4	TTTGCATTGTACAGCGAACG	GATATTCGCGGGTTCCTCAA	20	20	45.0	50.0	57.7	57.8	5.8	6.1	0.542647	117790	119202	1545-1545	1.000000	75.7
ndhD_p5	ndhD	5	TTGCATTGTACAGCGAACGA	ATTCGCGGGTTCCTCAATTT	20	20	45.0	45.0	58.5	58.2	0.0	6.1	0.662518	117790	119202	1541-1541	1.000000	74.7
ndhD-psaC_p1	ndhD-psaC	1	TGGATCGTCCAATTGGAAATGA	AACCGAGGACTGTGTAGGTT	22	20	40.9	50.0	58.0	58.3	28.6	0.0	2.324853	119203	119413	416-416	1.000000	62.7
ndhD-psaC_p2	ndhD-psaC	2	TGGATCGTCCAATTGGAAATGA	AAGAGATGTGAATCCGCCTG	22	20	40.9	50.0	58.0	57.7	28.6	0.0	2.360506	119203	119413	394-394	1.000000	62.5
ndhD-psaC_p3	ndhD-psaC	3	TGGATCGTCCAATTGGAAATGA	AGAACCGAGGACTGTGTAGG	22	20	40.9	55.0	58.0	58.5	28.6	0.0	2.491190	119203	119413	418-418	1.000000	61.4
ndhD-psaC_p4	ndhD-psaC	4	TGGATCGTCCAATTGGAAATGA	ATGAAACAACTCGCAGCATG	22	20	40.9	45.0	58.0	57.4	28.6	0.0	2.662467	119203	119413	336-336	1.000000	60.4
ndhD-psaC_p5	ndhD-psaC	5	TGGATCGTCCAATTGGAAATGA	CATGAAACAACTCGCAGCAT	22	20	40.9	45.0	58.0	57.4	28.6	0.0	2.662467	119203	119413	337-337	1.000000	60.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119660	120311	769-846	1.000000	78.6
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119660	120311	768-845	1.000000	78.6
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	119660	120311	789-866	1.000000	78.5
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	119660	120311	767-844	1.000000	77.5
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	119660	120311	744-821	1.000000	77.1
ycf1_p1	ycf1	1	TCGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACA	23	21	39.1	42.9	58.0	57.5	0.0	15.9	4.522366	127035	127184	306-306	1.000000	45.1
ycf1_p2	ycf1	2	TCGGTTCGACAATCAATATTCC	ACAATTACGCGAGATCAGACA	22	21	40.9	42.9	56.6	57.5	0.0	15.9	4.900784	127035	127184	306-306	1.000000	42.1
ycf1_p3	ycf1	3	CGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACA	22	21	40.9	42.9	56.6	57.5	0.0	15.9	4.909137	127035	127184	305-305	1.000000	42.0
ycf1_p4	ycf1	4	TCGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACAT	23	22	39.1	40.9	58.0	58.0	0.0	15.9	5.065477	127035	127184	306-306	1.000000	41.3
ycf1_p5	ycf1	5	TCGGTTCGACAATCAATATTCCT	GACAATTACGCGAGATCAGAC	23	21	39.1	47.6	58.0	56.9	0.0	15.9	5.110529	127035	127184	307-307	1.000000	41.3