Hotspot

rpl16

LSC · 86201-87714 · 1514 bp

Back to Hymenopus

Pi 0.00892

MarkerSeek score 53.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1514
variable_sites: 13
pi: 0.00892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AATTTTCGGGCTCCACTGTT	GCCGAATGGGTTAGAGAAGG	45.0	55.0	58.0	58.0	1959-2003 bp	1.0	82.8
rpl16_p2	AATTTTCGGGCTCCACTGTT	CAGGACGTCTTGACGGAAAA	45.0	50.0	58.0	58.1	1991-2035 bp	1.0	81.9
rpl16_p3	AATTTTCGGGCTCCACTGTT	GGACGTCTTGACGGAAAAGA	45.0	50.0	58.0	57.9	1989-2033 bp	1.0	81.9
rpl16_p4	AGAGGTGAATTTGGTACGGC	GCCGAATGGGTTAGAGAAGG	50.0	55.0	57.9	58.0	2058-2102 bp	1.0	81.8
rpl16_p5	GAGGTGAATTTGGTACGGCT	GCCGAATGGGTTAGAGAAGG	50.0	55.0	57.9	58.0	2057-2101 bp	1.0	81.8