Hotspot

trnR-ACG-trnN-GUU

IRb · 113943-114573 · 631 bp

Back to Hymenopus

Pi 0.00951

MarkerSeek score 64.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 631
variable_sites: 6
pi: 0.00951
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	772 bp	1.0	78.9
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	809 bp	1.0	78.3
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	813 bp	1.0	78.1
trnR-ACG-trnN-GUU_p4	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	826 bp	1.0	77.8
trnR-ACG-trnN-GUU_p5	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	827 bp	1.0	77.8