Hotspot

clpP-psbB

LSC · 76496-76976 · 481 bp

Back to Hymenopus

Pi 0.0146

MarkerSeek score 64.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 7
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ATTGAATCGTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	50.0	50.0	56.7	58.2	557-558 bp	1.0	68.9
clpP-psbB_p2	ATTGAATCGTCCTCTCCAGG	CAAACGACCGGGATCATTCA	50.0	50.0	56.7	58.3	594-595 bp	1.0	68.6
clpP-psbB_p3	ATTGAATCGTCCTCTCCAGG	AGCAACCAAAGCTGTATGCA	50.0	45.0	56.7	58.4	630-631 bp	1.0	67.7
clpP-psbB_p4	ATTGAATCGTCCTCTCCAGG	ACACGATACCAAGGCAAACC	50.0	50.0	56.7	58.5	560-561 bp	1.0	67.1
clpP-psbB_p5	ATTGAATCGTCCTCTCCAGG	AAACGACCGGGATCATTCAA	50.0	45.0	56.7	57.5	593-594 bp	1.0	66.9