Hotspot

rpl20-rps12

LSC · 73293-74116 · 824 bp

Back to Hymenopus

Pi 0.011

MarkerSeek score 65.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 9
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGCGCTATATATCCTCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	881-900 bp	1.0	78.5
rpl20-rps12_p2	TCCGCGCTATATATCCTCGT	AGGAACATGTACTCGGGTGT	50.0	50.0	58.0	58.4	880-899 bp	1.0	78.0
rpl20-rps12_p3	TCCGCGCTATATATCCTCGT	AAATGTCACCAAATCCCCCG	50.0	50.0	58.0	58.4	925-944 bp	1.0	77.3
rpl20-rps12_p4	AGTAAGTCTTGAATGCGCCC	GAGGAACATGTACTCGGGTG	50.0	55.0	58.3	57.7	943-962 bp	1.0	76.6
rpl20-rps12_p5	AGTAAGTCTTGAATGCGCCC	AGGAACATGTACTCGGGTGT	50.0	50.0	58.3	58.4	942-961 bp	1.0	76.1