Hotspot

petA-psbJ

LSC · 66719-67571 · 853 bp

Back to Hymenopus

Pi 0.0101

MarkerSeek score 67.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 8
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	47.6	55.0	58.0	57.8	951-1006 bp	1.0	74.7
petA-psbJ_p2	CCCATTACGTGTTCAAGGACT	GGGTCATATTCCGGATTGGG	47.6	55.0	57.9	57.8	949-1004 bp	1.0	74.6
petA-psbJ_p3	ACCCATTACGTGTTCAAGGAC	GGGTCATATTCCGGATTGGG	47.6	55.0	58.2	57.8	950-1005 bp	1.0	73.2
petA-psbJ_p4	ACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	45.0	55.0	56.8	57.8	950-1005 bp	1.0	73.2
petA-psbJ_p5	GACCCATTACGTGTTCAAGGA	GGTCATATTCCGGATTGGGC	47.6	55.0	58.0	58.5	950-1005 bp	1.0	72.5