Hotspot

trnF-GAA-ndhJ

LSC · 50662-51489 · 828 bp

Back to Hymenopus

Pi 0.00254
MarkerSeek score 65.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
828
variable_sites
2
pi
0.00254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA GAAAGTTGGATAGGGTGGCC 55.0 55.0 58.0 58.2 952-973 bp 1.0 81.7
trnF-GAA-ndhJ_p2 GCATCAGAAATGGTCGGGAT GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.0 58.2 967-988 bp 1.0 81.5
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.1 58.2 916-937 bp 1.0 81.5
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA GAAAGTTGGATAGGGTGGCC 55.0 55.0 57.9 58.2 940-961 bp 1.0 80.7
trnF-GAA-ndhJ_p5 ATGCATCAGAAATGGTCGGG GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.3 58.2 969-990 bp 1.0 79.2