Hotspot

trnT-GGU-psbD

LSC · 33524-35130 · 1607 bp

Back to Hymenopus

Pi 0.00962

MarkerSeek score 57.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1607
variable_sites: 15
pi: 0.00962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1746-1749 bp	1.0	82.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1749-1752 bp	1.0	82.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AAGCAATAGACCGGACCAAC	55.0	50.0	58.1	57.9	1776-1779 bp	1.0	81.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	AAGCAATAGACCGGACCAAC	50.0	50.0	58.1	57.9	1779-1782 bp	1.0	81.3
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1723-1726 bp	1.0	80.6