Hotspot

trnS-GCU-trnG-GCC

LSC · 7310-8255 · 946 bp

Back to Hymenopus

Pi 0.0127

MarkerSeek score 66.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 946
variable_sites: 12
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	AGGGATCCCTTAACTATTAGGCT	50.0	43.5	58.0	57.9	1046-1066 bp	1.0	56.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	AGGGATCCCTTAACTATTAGGCT	50.0	43.5	58.0	57.9	1045-1065 bp	1.0	56.2
trnS-GCU-trnG-GCC_p3	ATCAACGGAACCGGAAAGAG	AGGGATCCCTTAACTATTAGGCT	50.0	43.5	57.9	57.9	1110-1130 bp	1.0	55.4
trnS-GCU-trnG-GCC_p4	AGCAATCCGACGCTTTAGTC	AGGGATCCCTTAACTATTAGGCT	50.0	43.5	58.4	57.9	1048-1068 bp	1.0	53.3
trnS-GCU-trnG-GCC_p5	CGACGCTTTAGTCCACTCAG	AGGGATCCCTTAACTATTAGGCT	55.0	43.5	58.4	57.9	1041-1061 bp	1.0	53.2