Hotspot

trnV-GAC-rrn16

Genome · 98275-104001 · 5727 bp

Back to Hymenophyllum

Pi 0.277

MarkerSeek score 76.00

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 5727
variable_sites: 2145
pi: 0.277
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	TCCGCTTAACTCGTTGATGG	TGGACCTTTCACCAATGTGG	50.0	50.0	58.0	58.0	666 bp	0.2	47.9
trnV-GAC-rrn16_p2	GATGTAACCAGAAGCAGCGA	TGGACCTTTCACCAATGTGG	50.0	50.0	58.0	58.0	455 bp	0.2	47.9
trnV-GAC-rrn16_p3	TCCGCTTAACTCGTTGATGG	GCTTGGAGGAGACTGTAAGC	50.0	55.0	58.0	58.0	1019 bp	0.2	47.8
trnV-GAC-rrn16_p4	GATGTAACCAGAAGCAGCGA	GCTTGGAGGAGACTGTAAGC	50.0	55.0	58.0	58.0	808 bp	0.2	47.8
trnV-GAC-rrn16_p5	TCCGCTTAACTCGTTGATGG	TAAGCAAGATCTTGGCCGAC	50.0	50.0	58.0	58.0	1004 bp	0.2	47.8