Hotspot

psbE-petL

Genome · 69296-69964 · 669 bp

Back to Hymenophyllum

Pi 0.0528

MarkerSeek score 87.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 669
variable_sites: 71
pi: 0.0528
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCAGCAAAAGGACGTTCTCC	AGCAAAGTAACTTAGTAGAGTAAGCA	50.0	34.6	58.1	58.1	726-757 bp	1.0	51.7
psbE-petL_p2	TCAGCAAAAGGACGTTCTCC	GCAAAGTAACTTAGTAGAGTAAGCA	50.0	36.0	58.1	56.9	725-756 bp	1.0	51.7
psbE-petL_p3	TGTCAGCAAAAGGACGTTCT	AGCAAAGTAACTTAGTAGAGTAAGCA	45.0	34.6	57.7	58.1	728-759 bp	1.0	51.7
psbE-petL_p4	TGTCAGCAAAAGGACGTTCT	GCAAAGTAACTTAGTAGAGTAAGCA	45.0	36.0	57.7	56.9	727-758 bp	1.0	51.7
psbE-petL_p5	ACCAACCTGCAATGAACAGA	AGCAAAGTAACTTAGTAGAGTAAGCA	45.0	34.6	57.6	58.1	797-828 bp	1.0	51.0