Hotspot

rps4-trnL-TAA

Genome · 52557-53439 · 883 bp

Back to Hymenophyllum

Pi 0.0717

MarkerSeek score 85.70

Primer available yes

Label rps4-trnL-TAA

Feature Metadata

label_name: rps4-trnL-TAA
feature_type: igs
region: Genome
length_bp: 883
variable_sites: 118
pi: 0.0717
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-TAA_p1	AAAACGGGGTCCTCGATAAC	GCGTCTACCATTTCGCCATA	50.0	50.0	57.6	58.1	872-935 bp	1.0	87.8
rps4-trnL-TAA_p2	TTTTAAAACGGGGTCCTCGA	GCGTCTACCATTTCGCCATA	45.0	50.0	57.1	58.1	876-939 bp	1.0	83.5
rps4-trnL-TAA_p3	AAAACGGGGTCCTCGATAAC	TACCATTTCGCCATATCCCC	50.0	50.0	57.6	57.1	867-930 bp	1.0	81.9
rps4-trnL-TAA_p4	TTTAAAACGGGGTCCTCGAT	GCGTCTACCATTTCGCCATA	45.0	50.0	56.8	58.1	875-938 bp	1.0	81.6
rps4-trnL-TAA_p5	TTTTAAAACGGGGTCCTCGAT	GCGTCTACCATTTCGCCATA	42.9	50.0	57.6	58.1	876-939 bp	1.0	79.5