Hotspot

trnS-TGA-psbC

Genome · 38433-39199 · 767 bp

Back to Hymenophyllum

Pi 0.0706

MarkerSeek score 83.00

Primer available yes

Label trnS-TGA-psbC

Feature Metadata

label_name: trnS-TGA-psbC
feature_type: igs
region: Genome
length_bp: 767
variable_sites: 106
pi: 0.0706
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-TGA-psbC_p1	ATGGTCGAGTGGTTTATGGC	TCCCCCACAGGAGAAATCAT	50.0	50.0	58.0	58.0	1179-1243 bp	1.0	91.6
trnS-TGA-psbC_p2	ATGGTCGAGTGGTTTATGGC	CGCTAACATAGGATCCGCTC	50.0	55.0	58.0	58.0	1234-1298 bp	1.0	91.6
trnS-TGA-psbC_p3	ATGGTCGAGTGGTTTATGGC	GAAACTATGCGCTTCTGGGA	50.0	50.0	58.0	58.0	1149-1213 bp	1.0	91.5
trnS-TGA-psbC_p4	ATGGTCGAGTGGTTTATGGC	GATCTTCGTGCTCCATGGTT	50.0	50.0	58.0	58.0	1131-1195 bp	1.0	91.5
trnS-TGA-psbC_p5	ATGGTCGAGTGGTTTATGGC	GGTCCGGAGAAGCTTACTTG	50.0	55.0	58.0	58.0	1412-1476 bp	1.0	90.7