Hotspot

trnE-TTC-trnG-GCC

Genome · 36883-37479 · 597 bp

Back to Hymenophyllum

Pi 0.108

MarkerSeek score 84.40

Primer available yes

Label trnE-TTC-trnG-GCC

Feature Metadata

label_name: trnE-TTC-trnG-GCC
feature_type: igs
region: Genome
length_bp: 597
variable_sites: 131
pi: 0.108
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnG-GCC_p1	GTCGCCTCCTTGAAAGAGAG	GTTCGATTCCCGCTATCCG	55.0	57.9	58.0	58.2	666-1030 bp	1.0	80.5
trnE-TTC-trnG-GCC_p2	GTCGCCTCCTTGAAAGAGAG	GGGTTCGATTCCCGCTATC	55.0	57.9	58.0	57.8	668-1032 bp	1.0	80.3
trnE-TTC-trnG-GCC_p3	GTCGCCTCCTTGAAAGAGAG	GGTTCGATTCCCGCTATCC	55.0	57.9	58.0	57.8	667-1031 bp	1.0	80.3
trnE-TTC-trnG-GCC_p4	TCGCCTCCTTGAAAGAGAGA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	665-1029 bp	1.0	79.9
trnE-TTC-trnG-GCC_p5	TCGCCTCCTTGAAAGAGAGA	GGTTCGATTCCCGCTATCC	50.0	57.9	58.1	57.8	666-1030 bp	1.0	79.7