primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	psbK-psbI	1	CCATCCAGAGCTGAAAAGGG	TTCGAATCCCTCTCTCTCCC	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.314537	13871	14675	1582-1719	0.800000	80.3
psbK-psbI_p2	psbK-psbI	2	CCATCCAGAGCTGAAAAGGG	GGTTGGCCTTGAATTGAACG	20	20	55.0	50.0	58.2	57.9	0.0	3.3	0.304382	13871	14675	2207-2210	0.400000	56.0
psbK-psbI_p3	psbK-psbI	3	TGGAAGATCCATCCAGAGCT	GGTTGGCCTTGAATTGAACG	20	20	50.0	50.0	57.8	57.9	12.6	3.3	0.251812	13871	14675	2215-2215	0.200000	46.0
psbK-psbI_p4	psbK-psbI	4	TGGAAGATCCATCCAGAGCT	TTCGAATCCCTCTCTCTCCC	20	20	50.0	55.0	57.8	57.9	12.6	0.0	0.261968	13871	14675	1676-1676	0.200000	45.9
psbK-psbI_p5	psbK-psbI	5	TGGAAGATCCATCCAGAGCT	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.8	58.2	12.6	17.2	0.404168	13871	14675	1679-1679	0.200000	44.8
trnG-TCC_p1	trnG-TCC	1	GTCTCCGGAAACAGGCTAAG	GGTCAGCGTCTACGTGAATT	20	20	55.0	50.0	58.0	58.0	5.2	0.0	0.019370	15798	16850	1846-1875	1.000000	90.7
trnG-TCC_p2	trnG-TCC	2	GTCTCCGGAAACAGGCTAAG	GACAATGGACGCTCCTTCAT	20	20	55.0	50.0	58.0	58.0	5.2	5.8	0.041378	15798	16850	1383-1410	0.800000	82.4
trnG-TCC_p3	trnG-TCC	3	GAGTTCCGGAGATCAACACC	TGTTTACGGAACAAGCGGAA	20	20	55.0	45.0	58.0	58.0	3.3	4.4	0.060712	15798	16850	1608-1638	0.600000	73.1
trnG-TCC_p4	trnG-TCC	4	GAGTTCCGGAGATCAACACC	GGTCAGCGTCTACGTGAATT	20	20	55.0	50.0	58.0	58.0	3.3	0.0	0.019370	15798	16850	1810-1840	0.600000	72.5
trnG-TCC_p5	trnG-TCC	5	GAGTTCCGGAGATCAACACC	GACAATGGACGCTCCTTCAT	20	20	55.0	50.0	58.0	58.0	3.3	5.8	0.041378	15798	16850	1348-1375	0.400000	59.6
atpF-atpH_p1	atpF-atpH	1	CAAAACCCGCAGCAAGAATC	AGCATCATCATTTGCAAATCCC	20	22	50.0	40.9	58.3	57.9	0.0	21.7	2.366269	20015	20361	428-439	1.000000	71.9
atpF-atpH_p2	atpF-atpH	2	CAAAACCCGCAGCAAGAATC	GCATCATCATTTGCAAATCCCT	20	22	50.0	40.9	58.3	57.9	0.0	21.7	2.366269	20015	20361	427-438	1.000000	71.9
atpF-atpH_p3	atpF-atpH	3	CCCGCAGCAAGAATCCAATA	AGCATCATCATTTGCAAATCCC	20	22	50.0	40.9	58.3	57.9	0.0	21.7	2.388833	20015	20361	423-434	1.000000	71.8
atpF-atpH_p4	atpF-atpH	4	CCCGCAGCAAGAATCCAATA	GCATCATCATTTGCAAATCCCT	20	22	50.0	40.9	58.3	57.9	0.0	21.7	2.388833	20015	20361	422-433	1.000000	71.8
atpF-atpH_p5	atpF-atpH	5	CGCAGCAAGAATCCAATAGC	GCATCATCATTTGCAAATCCCT	20	22	50.0	40.9	57.6	57.9	0.0	21.7	2.464133	20015	20361	420-431	1.000000	71.3
atpH-atpI_p1	atpH-atpI	1	GCAGCAATAACAGAAGCAGC	GGAGAATCTATGGAAGGTCATCA	20	23	50.0	43.5	58.1	57.2	0.0	0.0	3.909784	20608	21215	670-711	1.000000	58.9
atpH-atpI_p2	atpH-atpI	2	GCAGCAATAACAGAAGCAGC	AGGAGAATCTATGGAAGGTCA	20	21	50.0	42.9	58.1	55.2	0.0	0.0	3.923684	20608	21215	671-712	1.000000	58.8
atpH-atpI_p3	atpH-atpI	3	GCAGCAATAACAGAAGCAGC	GCTGCAGCTTATATAGGAGA	20	20	50.0	45.0	58.1	54.1	0.0	31.3	3.972141	20608	21215	685-726	1.000000	58.2
atpH-atpI_p4	atpH-atpI	4	CCGATAGAAGCAAGGCCTAC	GGAGAATCTATGGAAGGTCATCA	20	23	55.0	43.5	57.8	57.2	5.2	0.0	3.971576	20608	21215	700-741	1.000000	58.0
atpH-atpI_p5	atpH-atpI	5	CCGATAGAAGCAAGGCCTAC	AGGAGAATCTATGGAAGGTCA	20	21	55.0	42.9	57.8	55.2	5.2	0.0	3.985476	20608	21215	701-742	1.000000	57.9
psbM-trnD-GTC_p1	psbM-trnD-GTC	1	TGCAGTAGCTATGAATGCGA	GGTGCTCTAACCGATTGAACT	20	21	45.0	47.6	57.1	58.0	14.5	0.0	1.914889	35834	36488	618-720	1.000000	76.7
psbM-trnD-GTC_p2	psbM-trnD-GTC	2	GTGCAGTAGCTATGAATGCG	GGTGCTCTAACCGATTGAACT	20	21	50.0	47.6	57.1	58.0	14.5	0.0	1.964050	35834	36488	619-721	1.000000	76.3
psbM-trnD-GTC_p3	psbM-trnD-GTC	3	GCAGTAGCTATGAATGCGAGA	GGTGCTCTAACCGATTGAACT	21	21	47.6	47.6	58.0	58.0	0.0	0.0	2.044653	35834	36488	617-719	1.000000	75.6
psbM-trnD-GTC_p4	psbM-trnD-GTC	4	TGCAGTAGCTATGAATGCGA	GGGATTGACGGGTTTCGAAC	20	20	45.0	55.0	57.1	59.2	14.5	0.0	2.088202	35834	36488	662-764	1.000000	75.3
psbM-trnD-GTC_p5	psbM-trnD-GTC	5	TGCAGTAGCTATGAATGCGA	GGGATTGACGGGTTTCGAA	20	19	45.0	52.6	57.1	57.8	14.5	0.0	2.125438	35834	36488	662-764	1.000000	75.0
trnE-TTC-trnG-GCC_p1	trnE-TTC-trnG-GCC	1	GTCGCCTCCTTGAAAGAGAG	GTTCGATTCCCGCTATCCG	20	19	55.0	57.9	58.0	58.2	0.0	0.0	1.192711	36883	37479	666-1030	1.000000	80.5
trnE-TTC-trnG-GCC_p2	trnE-TTC-trnG-GCC	2	GTCGCCTCCTTGAAAGAGAG	GGGTTCGATTCCCGCTATC	20	19	55.0	57.9	58.0	57.8	0.0	9.9	1.215646	36883	37479	668-1032	1.000000	80.3
trnE-TTC-trnG-GCC_p3	trnE-TTC-trnG-GCC	3	GTCGCCTCCTTGAAAGAGAG	GGTTCGATTCCCGCTATCC	20	19	55.0	57.9	58.0	57.8	0.0	0.0	1.215646	36883	37479	667-1031	1.000000	80.3
trnE-TTC-trnG-GCC_p4	trnE-TTC-trnG-GCC	4	TCGCCTCCTTGAAAGAGAGA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.1	58.2	9.0	0.0	1.264783	36883	37479	665-1029	1.000000	79.9
trnE-TTC-trnG-GCC_p5	trnE-TTC-trnG-GCC	5	TCGCCTCCTTGAAAGAGAGA	GGTTCGATTCCCGCTATCC	20	19	50.0	57.9	58.1	57.8	9.0	0.0	1.287719	36883	37479	666-1030	1.000000	79.7
trnS-TGA-psbC_p1	trnS-TGA-psbC	1	ATGGTCGAGTGGTTTATGGC	TCCCCCACAGGAGAAATCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052765	38433	39199	1179-1243	1.000000	91.6
trnS-TGA-psbC_p2	trnS-TGA-psbC	2	ATGGTCGAGTGGTTTATGGC	CGCTAACATAGGATCCGCTC	20	20	50.0	55.0	58.0	58.0	0.0	6.0	0.054414	38433	39199	1234-1298	1.000000	91.6
trnS-TGA-psbC_p3	trnS-TGA-psbC	3	ATGGTCGAGTGGTTTATGGC	GAAACTATGCGCTTCTGGGA	20	20	50.0	50.0	58.0	58.0	0.0	9.7	0.062562	38433	39199	1149-1213	1.000000	91.5
trnS-TGA-psbC_p4	trnS-TGA-psbC	4	ATGGTCGAGTGGTTTATGGC	GATCTTCGTGCTCCATGGTT	20	20	50.0	50.0	58.0	58.0	0.0	12.6	0.068225	38433	39199	1131-1195	1.000000	91.5
trnS-TGA-psbC_p5	trnS-TGA-psbC	5	ATGGTCGAGTGGTTTATGGC	GGTCCGGAGAAGCTTACTTG	20	20	50.0	55.0	58.0	58.0	0.0	3.1	0.049927	38433	39199	1412-1476	1.000000	90.7
psbD-trnT-GGT_p1	psbD-trnT-GGT	1	CTCTTCTTAGCCAATCGTCCA	GGCGTAAGTCATCGGTTCAA	21	20	47.6	50.0	57.5	58.3	0.0	0.0	1.809738	41631	43153	1481-1634	1.000000	77.5
psbD-trnT-GGT_p2	psbD-trnT-GGT	2	TCTTCTTAGCCAATCGTCCA	GGCGTAAGTCATCGGTTCAA	20	20	45.0	50.0	56.3	58.3	0.0	0.0	2.017668	41631	43153	1480-1633	1.000000	75.9
psbD-trnT-GGT_p3	psbD-trnT-GGT	3	TCTCTTCTTAGCCAATCGTCC	GGCGTAAGTCATCGGTTCAA	21	20	47.6	50.0	57.2	58.3	0.0	0.0	2.085755	41631	43153	1482-1635	1.000000	75.3
psbD-trnT-GGT_p4	psbD-trnT-GGT	4	CTCTTCTTAGCCAATCGTCCA	GCCATGGTAAGGCGTAAGTC	21	20	47.6	55.0	57.5	58.7	0.0	14.7	2.230204	41631	43153	1491-1644	1.000000	74.2
psbD-trnT-GGT_p5	psbD-trnT-GGT	5	CGATCTCTTCTTAGCCAATCGT	GGCGTAAGTCATCGGTTCAA	22	20	45.5	50.0	58.0	58.3	0.0	0.0	2.311656	41631	43153	1485-1638	1.000000	73.5
rps4-trnL-TAA_p1	rps4-trnL-TAA	1	AAAACGGGGTCCTCGATAAC	GCGTCTACCATTTCGCCATA	20	20	50.0	50.0	57.6	58.1	20.6	0.0	0.530940	52557	53439	872-935	1.000000	87.8
rps4-trnL-TAA_p2	rps4-trnL-TAA	2	TTTTAAAACGGGGTCCTCGA	GCGTCTACCATTTCGCCATA	20	20	45.0	50.0	57.1	58.1	20.6	0.0	1.066102	52557	53439	876-939	1.000000	83.5
rps4-trnL-TAA_p3	rps4-trnL-TAA	3	AAAACGGGGTCCTCGATAAC	TACCATTTCGCCATATCCCC	20	20	50.0	50.0	57.6	57.1	20.6	0.0	1.262712	52557	53439	867-930	1.000000	81.9
rps4-trnL-TAA_p4	rps4-trnL-TAA	4	TTTAAAACGGGGTCCTCGAT	GCGTCTACCATTTCGCCATA	20	20	45.0	50.0	56.8	58.1	20.6	0.0	1.295810	52557	53439	875-938	1.000000	81.6
rps4-trnL-TAA_p5	rps4-trnL-TAA	5	TTTTAAAACGGGGTCCTCGAT	GCGTCTACCATTTCGCCATA	21	20	42.9	50.0	57.6	58.1	20.6	0.0	1.561243	52557	53439	876-939	1.000000	79.5
psbE-petL_p1	psbE-petL	1	TCAGCAAAAGGACGTTCTCC	AGCAAAGTAACTTAGTAGAGTAAGCA	20	26	50.0	34.6	58.1	58.1	0.0	4.4	6.227389	69296	69964	726-757	1.000000	51.7
psbE-petL_p2	psbE-petL	2	TCAGCAAAAGGACGTTCTCC	GCAAAGTAACTTAGTAGAGTAAGCA	20	25	50.0	36.0	58.1	56.9	0.0	4.4	6.238781	69296	69964	725-756	1.000000	51.7
psbE-petL_p3	psbE-petL	3	TGTCAGCAAAAGGACGTTCT	AGCAAAGTAACTTAGTAGAGTAAGCA	20	26	45.0	34.6	57.7	58.1	0.0	4.4	6.431925	69296	69964	728-759	1.000000	51.7
psbE-petL_p4	psbE-petL	4	TGTCAGCAAAAGGACGTTCT	GCAAAGTAACTTAGTAGAGTAAGCA	20	25	45.0	36.0	57.7	56.9	0.0	4.4	6.443317	69296	69964	727-758	1.000000	51.7
psbE-petL_p5	psbE-petL	5	ACCAACCTGCAATGAACAGA	AGCAAAGTAACTTAGTAGAGTAAGCA	20	26	45.0	34.6	57.6	58.1	0.0	4.4	6.469649	69296	69964	797-828	1.000000	51.0
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	TCCGCTTAACTCGTTGATGG	TGGACCTTTCACCAATGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010627	101053	101172	666-666	0.200000	47.9
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	GATGTAACCAGAAGCAGCGA	TGGACCTTTCACCAATGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016189	101053	101172	455-455	0.200000	47.9
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	TCCGCTTAACTCGTTGATGG	GCTTGGAGGAGACTGTAAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021721	101053	101172	1019-1019	0.200000	47.8
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	GATGTAACCAGAAGCAGCGA	GCTTGGAGGAGACTGTAAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027283	101053	101172	808-808	0.200000	47.8
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	TCCGCTTAACTCGTTGATGG	TAAGCAAGATCTTGGCCGAC	20	20	50.0	50.0	58.0	58.0	0.0	31.4	0.028758	101053	101172	1004-1004	0.200000	47.8