Hotspot

atpF

LSC · 11867-13505 · 1639 bp

Back to Hymenocallis

Pi 0.0171

MarkerSeek score 67.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1639
variable_sites: 27
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCAGCTCGAA	GGCGAGCCAAGTGATACAAA	40.0	50.0	54.9	58.6	1761-1811 bp	1.0	54.3
atpF_p2	ACTAATTTCGTCAGCTCGAA	GGCGAGCCAAGTGATACAA	40.0	52.6	54.9	57.8	1761-1811 bp	1.0	49.5
atpF_p3	ACTAATTTCGTCAGCTCGAA	GCGAGCCAAGTGATACAAAA	40.0	45.0	54.9	56.7	1760-1810 bp	1.0	48.5
atpF_p4	ACTAATTTCGTCAGCTCGAA	AAAGGGCGAGCCAAGTGATA	40.0	50.0	54.9	59.4	1765-1815 bp	1.0	47.6
atpF_p5	TACTAATTTCGTCAGCTCGA	GGCGAGCCAAGTGATACAAA	40.0	50.0	54.0	58.6	1762-1812 bp	1.0	47.4