Hotspot

rpl2-rpl23

IRb · 88376-88410 · 35 bp

Back to Hymenocallis

Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 35
variable_sites: 1
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CACTTGACTGTCTACGGCTC	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.0	58.3	151 bp	1.0	78.3
rpl2-rpl23_p2	CCACAACGATGCTGTCCATA	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.0	58.3	198 bp	1.0	78.1
rpl2-rpl23_p3	CACTTGACTGTCTACGGCTC	ATCATTACGCTTCAACCGGG	55.0	50.0	58.0	58.3	158 bp	1.0	77.7
rpl2-rpl23_p4	CCACAACGATGCTGTCCATA	ATCATTACGCTTCAACCGGG	50.0	50.0	58.0	58.3	205 bp	1.0	77.5
rpl2-rpl23_p5	CTGTCTACGGCTCCATTACG	CGCTTCAACCGGGTTATTCT	55.0	50.0	57.9	58.3	144 bp	1.0	77.5