Hotspot

rpl33-rps18

LSC · 70196-70359 · 164 bp

Back to Hymenocallis

Pi 0.0187

MarkerSeek score 64.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TGTCGCTATTGTCACAAGCA	CAATTCGATCCCCCGATTCA	45.0	50.0	57.8	57.7	293-297 bp	1.0	78.6
rpl33-rps18_p2	TGTCGCTATTGTCACAAGCA	TCGATCCCCCGATTCAATTG	45.0	50.0	57.8	57.7	289-293 bp	1.0	78.6
rpl33-rps18_p3	TGTCGCTATTGTCACAAGCA	TCAATTCGATCCCCCGATTC	45.0	50.0	57.8	57.5	294-298 bp	1.0	76.2
rpl33-rps18_p4	TGTCGCTATTGTCACAAGCA	GGGAAAACGCCTACGAAAAG	45.0	50.0	57.8	57.4	268-272 bp	1.0	75.9
rpl33-rps18_p5	CGACACAATACACCCAGTCG	TCGATCCCCCGATTCAATTG	55.0	50.0	58.7	57.7	328-332 bp	1.0	74.4