Hotspot

petA-psbJ

LSC · 65317-66428 · 1112 bp

Back to Hymenocallis

Pi 0.0135

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 15
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGATTGGGT	50.0	47.6	57.8	57.8	1247-1251 bp	1.0	69.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	1289-1293 bp	1.0	69.5
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1288-1292 bp	1.0	69.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	GAAGGATTCCTCTTTGGCTGA	50.0	47.6	57.8	57.6	1321-1325 bp	1.0	68.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCT	50.0	45.0	57.8	56.6	1240-1244 bp	1.0	68.3