Hotspot

psbZ-trnG

LSC · 37199-37435 · 237 bp

Back to Hymenocallis

Pi 0.034

MarkerSeek score 65.60

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 8
pi: 0.034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	TGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	45.0	55.0	57.0	58.5	402-404 bp	1.0	70.3
psbZ-trnG_p2	TGCTTCTTCTGATGGTTGGT	GAACCCGCATCTTCTCCTTG	45.0	55.0	57.0	58.6	398-400 bp	1.0	69.3
psbZ-trnG_p3	GCTTCTTCTGATGGTTGGTCA	GATCGAACCCGCATCTTCTC	47.6	55.0	58.2	58.5	401-403 bp	1.0	68.2
psbZ-trnG_p4	TGCTTCTTCTGATGGTTGGTC	GATCGAACCCGCATCTTCTC	47.6	55.0	58.2	58.5	402-404 bp	1.0	68.2
psbZ-trnG_p5	TTGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	42.9	55.0	57.8	58.5	403-405 bp	1.0	68.1