Hotspot

petN-psbM

LSC · 29404-30400 · 997 bp

Back to Hymenocallis

Pi 0.00212

MarkerSeek score 64.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 2
pi: 0.00212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1105-1152 bp	1.0	72.7
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1054-1101 bp	1.0	71.9
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1053-1100 bp	1.0	71.9
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	1052-1099 bp	1.0	71.5
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1111-1158 bp	1.0	71.2