Hotspot

rps15-ycf1

SSC · 125561-125973 · 413 bp

Back to Hymenidium

Pi 0.0769

MarkerSeek score 79.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 104
pi: 0.0769
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACCCCTTGTTGTCTTCGTT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	58.0	58.5	936-1017 bp	0.761905	78.4
rps15-ycf1_p2	ATGAGTCGAAGAACACCGTG	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1324-1405 bp	0.761905	78.1
rps15-ycf1_p3	GGTCATTTCGTGTAGCTGGT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1266-1347 bp	0.761905	77.9
rps15-ycf1_p4	CCTAACTGCTCTGGTGCATT	GAGCCCATTCGTTTGTCTGT	50.0	50.0	57.9	58.5	1507-1588 bp	0.761905	77.7
rps15-ycf1_p5	GGTGCTGAGGACCCATATTG	GAGCCCATTCGTTTGTCTGT	55.0	50.0	58.0	58.5	1296-1377 bp	0.714286	76.5