Hotspot

trnT-GGU-psbD

LSC · 32829-34113 · 1285 bp

Back to Hymenidium

Pi 0.0531

MarkerSeek score 84.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 230
pi: 0.0531
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAATCAAACGGGCCCT	AAACTAGGCGCAAAGAACCA	45.0	45.0	58.1	58.0	1758-2055 bp	0.666667	77.8
trnT-GGU-psbD_p2	AATTGAATCAAACGGGCCCT	AACTAGGCGCAAAGAACCAA	45.0	45.0	58.1	58.0	1757-2054 bp	0.666667	77.8
trnT-GGU-psbD_p3	ATTGAATCAAACGGGCCCTT	AAACTAGGCGCAAAGAACCA	45.0	45.0	58.1	58.0	1757-2054 bp	0.666667	77.8
trnT-GGU-psbD_p4	ATTGAATCAAACGGGCCCTT	AACTAGGCGCAAAGAACCAA	45.0	45.0	58.1	58.0	1756-2053 bp	0.666667	77.8
trnT-GGU-psbD_p5	ATTGAATCAAACGGGCCCTT	ACCAAAACTAGGCGCAAAGA	45.0	45.0	58.1	58.0	1761-2058 bp	0.666667	77.8