Hotspot

psbM-trnD-GUC

LSC · 30747-31441 · 695 bp

Back to Hymenidium

Pi 0.0493

MarkerSeek score 81.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 117
pi: 0.0493
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGCAAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	26.1	50.0	52.7	58.5	543-807 bp	1.0	52.0
psbM-trnD-GUC_p2	AATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	26.1	50.0	52.4	58.5	542-806 bp	1.0	52.0
psbM-trnD-GUC_p3	AAATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	25.0	50.0	53.3	58.5	543-807 bp	1.0	52.0
psbM-trnD-GUC_p4	AAATGCAAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	26.1	50.0	52.7	57.0	544-808 bp	1.0	52.0
psbM-trnD-GUC_p5	AATGCAAGAATATTTACTTCCAT	GTTCAATTGGTCAGAGCACC	26.1	50.0	52.4	57.0	543-807 bp	1.0	52.0