Hotspot

atpH-atpI

LSC · 14200-15355 · 1156 bp

Back to Hymenidium

Pi 0.0489

MarkerSeek score 82.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1156
variable_sites: 189
pi: 0.0489
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTCTAATTGGTCAGGAGCGC	45.0	50.0	58.0	58.0	1213-1646 bp	1.0	91.5
atpH-atpI_p2	CTCGCGATACCTTCTACAGC	TTCTAATTGGTCAGGAGCGC	55.0	50.0	58.0	58.0	1303-1736 bp	0.952381	89.6
atpH-atpI_p3	CTTGGACTACGAACGGGAAG	TTCTAATTGGTCAGGAGCGC	55.0	50.0	58.0	58.0	1620-2053 bp	0.714286	79.8
atpH-atpI_p4	CTTGGACTACGAACGGGAAG	CTAATTGGTCAGGAGCGCTT	55.0	50.0	58.0	58.0	1618-2051 bp	0.714286	79.8
atpH-atpI_p5	CTTGGACTACGAACGGGAAG	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1527-1960 bp	0.666667	77.9