Hotspot

rps16-trnQ-UUG

LSC · 6344-7675 · 1332 bp

Back to Hymenidium

Pi 0.0739
MarkerSeek score 83.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1332
variable_sites
280
pi
0.0739
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCTACCCGAGCTCCATCAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1343-2319 bp 1.0 90.8
rps16-trnQ-UUG_p2 GATTCCCCGCGATACACTTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 2234-2334 bp 0.238095 54.9
rps16-trnQ-UUG_p3 GATTCCCCGCGATACACTTT TTTGCCCTGGAATTGACCAA 50.0 45.0 58.1 57.9 1779-1872 bp 0.238095 54.8
rps16-trnQ-UUG_p4 GATTCCCCGCGATACACTTT CCCAGCTATCCAACGAAGAC 50.0 55.0 58.1 58.1 2380-2460 bp 0.142857 50.1
rps16-trnQ-UUG_p5 TTCTACCCGAGCTCCATCAT CCCAGCTATCCAACGAAGAC 50.0 55.0 57.9 58.1 2193-2272 bp 0.142857 49.7