Hotspot

trnK-UUU-rps16

LSC · 4349-5236 · 888 bp

Back to Hymenidium

Pi 0.069
MarkerSeek score 80.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
888
variable_sites
205
pi
0.069
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 918-1300 bp 0.857143 85.6
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 917-1299 bp 0.857143 85.6
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG AAAGTGTATCGCGGGGAATC 55.0 50.0 58.0 58.1 1732-1766 bp 0.238095 53.9
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG AAAGTGTATCGCGGGGAATC 55.0 50.0 58.0 58.1 1731-1765 bp 0.238095 53.9
trnK-UUU-rps16_p5 TCCCTCGCTTCATCCAAATG AAAGTGTATCGCGGGGAATC 50.0 50.0 58.0 58.1 1680-1714 bp 0.238095 53.8