Hotspot

trnH-GUG-psbA

LSC · 79-472 · 394 bp

Back to Hymenidium

Pi 0.109

MarkerSeek score 73.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 120
pi: 0.109
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	55.0	45.0	58.1	56.2	199-444 bp	1.0	76.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	213-458 bp	1.0	76.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	212-457 bp	1.0	76.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	214-459 bp	1.0	76.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	55.0	42.9	58.1	57.0	200-445 bp	1.0	74.7