Hotspot

rps16

LSC · 3427-4525 · 1099 bp

Back to Hymenasplenium

Pi 0.0435

MarkerSeek score 81.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1099
variable_sites: 149
pi: 0.0435
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CTTCTTCCTCAGCACTCGAC	GGGGGCATGATACCAAAGAG	55.0	55.0	58.0	58.0	2099-2118 bp	0.416667	64.7
rps16_p2	CTTCTTCCTCAGCACTCGAC	TTCCTCTGGGATATCGACCC	55.0	55.0	58.0	58.0	2210-2229 bp	0.416667	64.4
rps16_p3	CTTCTTCCTCAGCACTCGAC	CCTCTGGGATATCGACCCTT	55.0	55.0	58.0	58.0	2208-2227 bp	0.416667	64.4
rps16_p4	CACTCGACGGGATAGTACCT	TTCCTCTGGGATATCGACCC	55.0	55.0	58.0	58.0	2198-2203 bp	0.25	51.7
rps16_p5	CACTCGACGGGATAGTACCT	GGGGGCATGATACCAAAGAG	55.0	55.0	58.0	58.0	2087-2092 bp	0.25	51.7