Hotspot

rrn16S-trnI-GAU

IRa · 143542-143849 · 308 bp

Back to Hymenasplenium

Pi 0.0621

MarkerSeek score 80.50

Primer available yes

Label rrn16S-trnI-GAU

Feature Metadata

label_name: rrn16S-trnI-GAU
feature_type: igs
region: IRa
length_bp: 308
variable_sites: 45
pi: 0.0621
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S-trnI-GAU_p1	AGTGACTGGAGTGAAGTCGT	CTACCGCTGAGCTAATAGCC	50.0	55.0	58.3	57.6	380-394 bp	1.0	86.6
rrn16S-trnI-GAU_p2	AAGTCGTAACAAGGTAGCCG	CTACCGCTGAGCTAATAGCC	50.0	55.0	57.6	57.6	367-381 bp	1.0	86.3
rrn16S-trnI-GAU_p3	GGCTAGTGACTGGAGTGAAG	CTACCGCTGAGCTAATAGCC	55.0	55.0	57.3	57.6	384-398 bp	1.0	83.7
rrn16S-trnI-GAU_p4	GGGCTAGTGACTGGAGTGAA	CTACCGCTGAGCTAATAGCC	55.0	55.0	58.7	57.6	385-399 bp	1.0	83.1
rrn16S-trnI-GAU_p5	AGTGACTGGAGTGAAGTCGT	GAAGTTTCCACAGTCCAGGC	50.0	55.0	58.3	58.8	428-442 bp	1.0	82.9