Hotspot

psaC-ndhE

SSC · 114503-114847 · 345 bp

Back to Hymenasplenium

Pi 0.0699

MarkerSeek score 79.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 345
variable_sites: 74
pi: 0.0699
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTACAGCCGATACACGTGTC	CGAACTGGGAGATCTACTCG	55.0	55.0	58.5	57.0	435-455 bp	1.0	77.0
psaC-ndhE_p2	CAGCCGATACACGTGTCATA	CGAACTGGGAGATCTACTCG	50.0	55.0	57.5	57.0	432-452 bp	1.0	76.8
psaC-ndhE_p3	TACAGCCGATACACGTGTCA	CGAACTGGGAGATCTACTCG	50.0	55.0	58.8	57.0	434-454 bp	1.0	74.0
psaC-ndhE_p4	GGTACAGCCGATACACGTG	CGAACTGGGAGATCTACTCG	57.9	55.0	58.0	57.0	436-456 bp	1.0	72.3
psaC-ndhE_p5	CAGCCGATACACGTGTCATAT	CGAACTGGGAGATCTACTCG	47.6	55.0	58.0	57.0	437 bp	0.083333	26.8