Hotspot

trnN-GUU-rpl21

SSC · 106555-109552 · 2998 bp

Back to Hymenasplenium

Pi 0.0448

MarkerSeek score 76.50

Primer available yes

Label trnN-GUU-rpl21

Feature Metadata

label_name: trnN-GUU-rpl21
feature_type: igs
region: SSC
length_bp: 2998
variable_sites: 390
pi: 0.0448
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl21_p1	AAGGAGAGAGATCGGGATGG	TTTATCTCGCTGTTGCCTCC	55.0	50.0	58.0	58.0	1444 bp	0.5	64.7
trnN-GUU-rpl21_p2	AAGGAGAGAGATCGGGATGG	TATCGGCTCTTATTCACGCG	55.0	50.0	58.0	58.0	1512 bp	0.5	64.4
trnN-GUU-rpl21_p3	AAGGAGAGAGATCGGGATGG	GTTAGGTGCTACACTGGCTC	55.0	55.0	58.0	58.0	1382 bp	0.416667	60.4
trnN-GUU-rpl21_p4	AAGGAGAGAGATCGGGATGG	GGCGTACCTCCATTCTCTTG	55.0	55.0	58.0	58.1	1072 bp	0.416667	60.2
trnN-GUU-rpl21_p5	AAGGAGAGAGATCGGGATGG	TGCTTCCCAATCTGCTTTGT	55.0	45.0	58.0	58.0	1280 bp	0.333333	57.5