Hotspot

rpl33-rpl20

LSC · 64707-65259 · 553 bp

Back to Hymenasplenium

Pi 0.0464

MarkerSeek score 79.30

Primer available yes

Label rpl33-rpl20

Feature Metadata

label_name: rpl33-rpl20
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 68
pi: 0.0464
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rpl20_p1	CTTCCCAGACGCTTTGCTAT	TTGCGCAAGTAGCTACTCTG	50.0	50.0	58.0	58.0	1090-1091 bp	0.25	51.1
rpl33-rpl20_p2	CTTCCCAGACGCTTTGCTAT	TCGTTCGGATTAATGCAGCA	50.0	45.0	58.0	58.0	1189-1190 bp	0.25	51.0
rpl33-rpl20_p3	CTTCCCAGACGCTTTGCTAT	TGCCTACTGTTTCTCCAGGA	50.0	50.0	58.0	58.0	1068-1069 bp	0.25	51.0
rpl33-rpl20_p4	GCGTAAAAATCGTCGCAACA	TTGCGCAAGTAGCTACTCTG	45.0	50.0	58.1	58.0	724 bp	0.25	50.9
rpl33-rpl20_p5	GCGTAAAAATCGTCGCAACA	TCGTTCGGATTAATGCAGCA	45.0	45.0	58.1	58.0	823 bp	0.25	50.7