Hotspot

ccsA-petG

LSC · 61919-63091 · 1173 bp

Back to Hymenasplenium

Pi 0.0404

MarkerSeek score 81.60

Primer available yes

Label ccsA-petG

Feature Metadata

label_name: ccsA-petG
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 131
pi: 0.0404
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-petG_p1	AAATATTCGTTTGGGCGGGG	CCGATAGTGATGCTTCAACCA	50.0	47.6	58.9	58.1	1218-1246 bp	1.0	75.4
ccsA-petG_p2	AAATATTCGTTTGGGCGGGG	TCCCGATAGTGATGCTTCAAC	50.0	47.6	58.9	57.8	1220-1248 bp	1.0	74.7
ccsA-petG_p3	AATATTCGTTTGGGCGGGG	CCGATAGTGATGCTTCAACCA	52.6	47.6	58.2	58.1	1217-1245 bp	1.0	73.0
ccsA-petG_p4	AATATTCGTTTGGGCGGGG	TCCCGATAGTGATGCTTCAAC	52.6	47.6	58.2	57.8	1219-1247 bp	1.0	72.3
ccsA-petG_p5	AAATATTCGTTTGGGCGGGG	TCCCGATAGTGATGCTTCAA	50.0	45.0	58.9	56.3	1220-1248 bp	1.0	70.9