Hotspot

psbD-trnT-GGU

LSC · 33960-35171 · 1212 bp

Back to Hymenasplenium

Pi 0.0572

MarkerSeek score 83.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 200
pi: 0.0572
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AAAGGTTGTACCCGTGAACC	AAGGCATTAACTAAGCGGGG	50.0	50.0	58.0	57.9	2013-2055 bp	1.0	89.8
psbD-trnT-GGU_p2	GTGACAAAGGTTGTACCCGT	AAGGCATTAACTAAGCGGGG	50.0	50.0	58.0	57.9	2018-2060 bp	1.0	89.7
psbD-trnT-GGU_p3	TGACAAAGGTTGTACCCGTG	AAGGCATTAACTAAGCGGGG	50.0	50.0	58.0	57.9	2017-2059 bp	1.0	89.7
psbD-trnT-GGU_p4	CAGGACCCCACAATAGAAGC	AAGGCATTAACTAAGCGGGG	55.0	50.0	58.0	57.9	2137-2179 bp	1.0	89.5
psbD-trnT-GGU_p5	GCTTCAGGACCCCACAATAG	AAGGCATTAACTAAGCGGGG	55.0	50.0	58.0	57.9	2141-2183 bp	1.0	89.5