Hotspot

trnC-GCA-trnG-GCC

LSC · 29918-30358 · 441 bp

Back to Hymenasplenium

Pi 0.0654
MarkerSeek score 80.80
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
LSC
length_bp
441
variable_sites
82
pi
0.0654
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 486-512 bp 1.0 80.9
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 488-514 bp 1.0 80.7
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 487-513 bp 1.0 80.7
trnC-GCA-trnG-GCC_p4 TCCGTCTTACCGCTTGACTA GTTCGATTCCCGCTATCCG 50.0 57.9 58.2 58.2 474-500 bp 1.0 80.4
trnC-GCA-trnG-GCC_p5 TCCGTCTTACCGCTTGACTA GGTTCGATTCCCGCTATCC 50.0 57.9 58.2 57.8 475-501 bp 1.0 80.2