Hotspot

trnE-UUC-petN

LSC · 27836-29160 · 1325 bp

Back to Hymenasplenium

Pi 0.0623

MarkerSeek score 87.00

Primer available yes

Label trnE-UUC-petN

Feature Metadata

label_name: trnE-UUC-petN
feature_type: igs
region: LSC
length_bp: 1325
variable_sites: 224
pi: 0.0623
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-petN_p1	GTCCTGGGCCTCTAAACGAT	TGTTTTCCCCACCCCAATTT	55.0	45.0	59.2	57.8	1361-1401 bp	0.916667	77.1
trnE-UUC-petN_p2	TCCTGGGCCTCTAAACGATG	TGTTTTCCCCACCCCAATTT	55.0	45.0	59.2	57.8	1360-1400 bp	0.916667	77.1
trnE-UUC-petN_p3	TCCTGGGCCTCTAAACGAT	TGTTTTCCCCACCCCAATTT	52.6	45.0	57.7	57.8	1360-1400 bp	0.916667	76.0
trnE-UUC-petN_p4	CCTGGGCCTCTAAACGATG	TGTTTTCCCCACCCCAATTT	57.9	45.0	57.6	57.8	1359-1399 bp	0.916667	75.4
trnE-UUC-petN_p5	CTGGGCCTCTAAACGATGG	TGTTTTCCCCACCCCAATTT	57.9	45.0	57.6	57.8	1358-1398 bp	0.916667	75.4